Genetics and Molecular Biotechnology Expertise Group SITH ITB - Genetics and Molecular Biotechnology Expertise Group SITH ITB

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The research group of Genetics and Molecular Biotechnology (KK GBM) expertise actively participates in the Tridarma of higher education, with a focus on genetics and molecular biology. The development of science is carried out comprehensively from up to downstream in response to the dynamics of scientific and technological advances which include: exploration and conservation of genetic diversity, analysis of gene and protein functions, genetic engineering, synthetic biology, multi-omics (genomics, transcriptomics, nutrigenomics, etc.) which supported by bioinformatics and big data analysis.

The Genetics and Molecular Biotechnology Research Group consists of lecturers, academic and research assistants, post-doctoral and students, who are actively involved in mono and multidisciplinary studies at national and international institutions. Members of KK GBM also contribute to community service activities and cooperation with various industries. In 2020, members of KK GBM will take part in the research, testing, and control of COVID-19 at both the municipal and provincial levels. Main Tridarma activities in KK GBM include (1) Food and Agriculture Security; (2) Health and medical technology; and (3) Green technology.

Various awards have been received by the KK GBM, such as participation in various government and industry agencies as the expert team, the Indonesian National Research Council (DRN), the Indonesian Young Scientist Academy (ALMI), the Indonesian Young Researchers Forum (ForMIND), Indonesia Biogeography & Biodiversity (INABIG) Research Institute and the National Consortium for Vaccines. Also, KK GBM received various prestigious awards, including national and international outstanding lecturers, such as L’oreal Unesco for Women in Sciences, Indonesia Science and Technology, ITB Innovation Award, Schlumberger Faculty for the Future, Australia Endeavor Research, Scholarships Fullbright Research, The Award for Special Achievement of the Japanese Society for Biological Sciences in Space, and Rispro LPDP. Students of KK GBM members have also won several competitions, namely Indofood Research Nugraha, International Biology Olympiad (IBO), and Synthetic Biology (iGEM). Contributions to knowledge are presented in peer-reviewed papers, lay articles, and textbooks. The following are the patents and publications of the members of the KK GBM research group :

PATENT

Granted

  1. IDP000067777  :  Promoter gen eukaryotic elongation factor 1 alpha hasil isolasi dari tanaman Manihot esculenta Crabts CV. Adira (MeEF1A6)
  2. IDP000051217 : Metode Pengaturan Pematangan Buah Pisang Ambon Lumut (Musa acuminata cv. Pisang ambon lumut, AAA group) dengan Manipulasi Gen yang Berperan dalam Pengaturan Pematangan
  3. IDP000065610 : Promoter Gen Elongation Factor 1 alpha-3 (MeEF1alpha3) dari tanaman singkong (Manihot esculenta C.) kultivar Adira
  4. IDP000064225 : Metode meningkatkan ekspresi gen ADS (Amorfa 4,11-diena sintase) pada tanaman anuma (Artemisia annua L.) untuk pengingkatan kadar artemisin pada tanaman tersebut
  5. IDP000056160 : Proses isolasi dan kloning gen Tuf dari tebu (Saccharum officinarum)
  6. IDP000066410 : Vektor ekspresi untuk produksi berlebih hepatitis B surface antigen (HbsAg) pada Hansenula polymorpha

Registered

  1. P00202102679 : Pakan Ikan yang Terbuat dari Pisang Tongka Langit (Musa troglodytarum) untuk Meningkatkan Asupan Mikronutrien dan Sistem Imun Ikan.
  2. S00201708460 : Alat untuk mengatur pematangan buah dari material bambu yang dikombinasikan dengan material kimiawi pemecah etilen. Fruit Storage Chamber to control fruit ripening
  3. P00201809117: Metode Penghantaran Plasmid Antiviral Penghasil dsRNA Terenkapsulasi Kitosan pada Krustasea Melalui Pakan Hidup Alami Untuk Melawan White Spot Syndrome Virus (WSSV) Pada Udang
  4. P00201810374 : Produk Bakteri Escherichia coli yang Dimodifikasi secara Genetik Untuk Penapisan Cepat Kandidat Obat HIV (Human Immunodeficiency Virus) Baru
  5. P00201810375 : Metode Seleksi Kandidat Senyawa Penghambat Pembentukan Dimer Protease HIV-1
  6. P00201800256 : Kit Diagnostik Untuk Mendeteksi Infeksi Virus Hepatitis B Menggunakan Antibodi Primer lgY Anti Hepatitis B Surface
  7. P00201805761: Antigen (HBsAg) dan Antibodi Sekunder Monoklonal Anti-HBsAG Yang Terkonjugasi Horseradish Peroxidase (HRP) dan Proses Pembuatannya
  8. P00201805761  : Proses Pembuatan :  Mutan H. polymorpha untuk produksi berlebih Hepatitis B surface antigen (HBsAg)
  9. P00201809524 : Barcode DNA dan Metode untuk Mendeteksi Keberadaan Daging Babi dalam Produk Olahan Bakso Menggunakan Sekuen Primer Forward F3 dan Reverse B3 dari Gen Cytochrome Oxydase I
  10. P00201804204 : Pepper Yellow Leaf Curl Virus (PYLCV) asli Indonesia pada tanaman cabai dan plasmid rekombinan pembawa genom bipartit PYLCV serta metode seleksi tanaman cabai yang resisten terhadap serangan virus PYLCV tersebut
  1. P002020015556: Plasmid rekombinan dan sel inang escherichia coli rekombinan penghasil dirhamnolipid serta metoda produksi dirhamnolipid dengan komposisi media ekspresi yang optimum menggunakan plasmid dan sel inang tersebut dalam menghasilkan varian dirhamnolipid sebagai biosurfaktan.
  2. P00202000545: Barcode DNA dan metode multiplex PCR untuk mendeteksi DNA tikus (Rattus norvegicus dan Rattus argentiventer) dengan menggunakan barcode dna dari gen ATP synthase subunit 6

INTERNATIONAL PUBLICATION

2021

  1. Fenny Martha Dwivany*, Nisrina Sukriandi, Karlia Meitha, Tatas H.P. Brotosudarmo. (2021). In silico Characterization of the Structure of Genes and Proteins related to β-carotene Degradation in Musa acuminata‘ DH Pahang’ and Musa balbisiana‘ Pisang Klutuk Wulung’. Pertanika Journal of Tropical Agriculture Science Vol 44 (2) p 429-447. https://doi.org/10.47836/pjtas.44.2.10
  2. Fenny Martha Dwivany*, Karlia Meitha, Kuswati, Rizkita R. Esyanti, Husna Nugrahapraja. (2021). Chitosan improving Growth in Chili (Capsicum annuum L.) Plants and acting through Distinct Gene Regulation between Cultivars. Research Journal of Biotechnology Vol. 16 (2) p 87-92.
  3. Husna Nugrahapraja, Ervina Putri Abdullah, Fenny Martha Dwivany*. (2021). Genome-wide Identification and Characterization of the Pectin Methylesterase (PME) and Pectin Methylesterase Inhibitor (PMEI) Gene Family in the Banana A-genome (Musa acuminata) and B-genome (Musa balbisiana). Research Journal of Biotechnology Vol. 16 (2) p 87-92.
  4. Lulu’ Nisrina, Yunus Effendi, Adi Pancoro*. (2021). Revealing the role of Plant Growth Promoting Rhizobacteria in suppressive soils against Fusarium oxysporum f.sp. cubense based on metagenomic analysis. Heliyon 7(8) e07636. https://doi.org/10.1016/j.heliyon.2021.e07636

2020

  1. Fenny Martha Dwivany*, Giasintha Stefani, Agus Sutanto, Husna Nugrahapraja, Ketut Wikantika, Adriana Hiariej, Topik Hidayat, I. Nyoman Rai, Nisrina Sukriandi. (2020). Genetic Relationship between Tongka Langit Bananas (Musa troglodytarum L.) from Galunggung and Maluku, Indonesia, Based on ITS2. HAYATI Journal of Biosciences. Vol 27 (3) p 258-265.
  2. Cindy Novianti and Fenny Martha Dwivany*. (2020). Chitosan-based Edible Coating Prolongs Musa troglodytarum L. (‘Pisang Tongkat Langit’) Fruit Shelf-life and Changes the ACS1 and ACO1 Gene Expression Profile. Pertanika Journal of Tropical Agricultural Science 43 (4).
  3. Fenny Martha Dwivany*,  Muhammad Rifki Ramadhan, Carolin Lim, Agus Sutanto, Husna Nugrahapraja, Ketut Wikantika, Sigit Nur Pratama, Karlia Meitha and Aksarani Sa Pratiwi. (2020). Bali Bananas (Musa spp. L.) Genetic Relationship Based on Internal Transcribed Spacer 2 (ITS-2). Pertanika Journal of Tropical Agricultural Science 43 (4).
  4. Fenny M. Dwivany*, Veinardi Suendo, Ayesha Nilam, Nisrina Sukriandi. (2020). Carrageenan Edible Coating Application Prolongs Cavendish Banana Shelf Life. International Journal of Food Science. Volume 2020, Article ID 8861610, 11 pages. https://doi.org/10.1155/2020/8861610
  5. Fenny Martha Dwivany*, Kristi Lenci Patty and Cindy Novianti. (2020). Isolation and characterization of pisang tongkat langit (Musa troglodytarum L.) ACS1 and ACO1 gene expression during fruit ripening process. Malaysian Applied Biology 49(2), p 81-92. https://mabjournal.com/index.php?option=com_content&view=article&id=1016&catid=59:current-view&Itemid=56
  6. Muhammad Abdul Aziz, Rizkita Rachmi Esyanti, Karlia Meitha, Fenny M. Dwivany, Hany Husnul Chotimah. (2020). Chitosan suppresses the expression level of WRKY17 on red chili (Capsicum annuum) plant under drought stress. Indonesian Journal of Biotechnology Vol 25 (1) p 52-60. https://doi.org/10.22146/ijbiotech.49506
  7. Karlia Meitha, Fenny M. Dwivany*, Intan Fatmawati, Agus Sutanto, Sigit Nur Pratama, Husna Nugrahapraja, Ketut Wikantika. (2020). Phylogenetic analysis of 23 accessions of Indonesian banana cultivars based on Internal Transcribed Spacer 2 (ITS2) region. Indonesian Journal of Biotechnology Vol 25 (1) p 1-11. https://doi.org/10.22146/ijbiotech.55016
  8. Fenny M. Dwivany*, Fidya Syam, Husna Nugrahapraja, Ocky Karna Radjasa, Maelita Ramdani Moeis, Susumu Uchiyama. (2020). Dataset of microbial community structure in alcohol sprayed banana associated with ripening process. Data in Brief 29, 105216, p 1-7. https://doi.org/10.1016/j.dib.2020.105216
  9. Fenny M. Dwivany*, Husna Nugrahapraja, Aisha Rizky Rahmawati, Dwianty Putri Meitasari, Audie Masola Putra, Popi Septiani, Nadya Farah, Siti Subandiyah. (2020). Data on banana transcriptome in response to blood disease infection. Data in brief 29, 105133, p 1-4. https://doi.org/10.1016/j.dib.2020.105133
  10. Fenny M. Dwivany*, Husna Nugrahapraja, Eiichiro Fukusaki, Sastia Prama Putri, Cindy Novianti, Septhy Kusuma Radjasa, Tessa Fauziah, Lutfi Dewi Nirmala Sari. (2020). Dataset of Cavendish banana transcriptome in response to chitosan coating application. Data in Brief 29, 105337, p 1-5. https://doi.org/10.1016/j.dib.2020.105337
  11. Ahmedz Widiasta, Yunia Sribudiani, Husna Nugrahapraja, Dany Hilmanto, Nanan Sekarwana, Dedi Rachmadi. (2020). Potential role of ACE2-related microRNAs in COVID-19-associated nephropathy. Non-coding RNA Research, Volume 5, Issue 4, December 2020, pp 153-166. https://doi.org/10.1016/j.ncrna.2020.09.001
  12. Muhammad Yusuf Abduh, Enjelina Nababan, Firdanta Ginting, Jenny Juliati, Husna Nugrahapraja. (2020). Biodelignification of Lemon Peels Using Aspergillus sp. to Improve Yield and Composition of Extxracted Lemon Oil. IIUM Engineering Journal, Vol. 21, No. 2, pp 55-66. https://doi.org/10.31436/iiumej.v21i2.1320
  13. Annisa Amalia, Husna Nugrahapraja*. (2020). The Assessment on RNA Quality and Banana Ripening Genes (MaGAPDH, MaACS1, And MaACO1) from Flesh Tissues with Different Preservation Methods. Malaysian Applied Biology, 49(2), pp 93–97. https://mabjournal.com/index.php?option=com_content&view=article&id=1017&catid=59:current-view&Itemid=56
  14. Gita Widya Pradini, Edhyana Sahiratmadja, Sony Suhandono, Sunarjati Sudigdoadi , Muhammad Yusuf, Ade Rizqi Ridwan Firdaus & Herman Susanto. (2020). Phylogeny and In Silico Structure Analysis of Major Capsid Protein (L1) Human Papillomavirus 45 from Indonesian Isolates. Asian Pac J Cancer Prev,21 (9), 2517-252. DOI:10.31557/APJCP.2020.21.9.2517
  1. Elfahmi Elfahmi, Fanny Mutia Cahyani, Tati Kristianti, Sony Suhandono. (2020). Tranformation of amorphadine synthase and antisilencing P19 genes into Artemisia annua L. And its effect on antimalarial artemisinin production. Advanced pharmaceutical bulletin. 10(3), 464-471.
  2. Rifqi Z Janatunaim, Azzania Fibriani*. (2020). Construction and Cloning of Plastic-degrading Recombinant Enzymes (MHETase). Recent Patents on Biotechnology, Volume 14 , Issue 3, pp 229-234. https://doi.org/10.2174/1872208314666200311104541
  3. Tafroji, W., Bernadette, F.M., Giri Rachman, E.A., Safari, D. (2020). The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae. Journal of Microbiological Methods. Vol. 172, 105909. https://doi.org/10.1016/j.mimet.2020.105909

NATIONAL PUBLICATION

2020

  1. Hayati Minarsih, Sony Suhandono, Annisa K. Fuadi, Tati Kristianti, Riza A. Putranto, Deden Sukmadjaya & Sustriprijatno. (2020). Isolation and characterization of Dehydrin promoter region from sugarcane (Saccharum officinarum L.). Menara perkebunan, 88(1), 16 -28.
  2. Hayati Minarsih Iskandar, Sony Suhandono, Jember Pambudi, Tati Kristianti, Riza A. Putranto, windi Mose and Sustiprijatno. (2020). Characterization of a drought inducible Dehydrin promoter from sugarcane (Saccharum officinarum L.) in tobacco (Nicotiana tabacum L.). Journal of Tropical Crops Science, 7(1), 27-36

PROCEEDING

2020

  1. Afifatul Achyar, Tati Kristianti, Sony Suhandono*. (2020). Fusion Strategy of Influenza A-H5N1 Virus M2e Epitope DNA Sequence to Hepatitis B Virus HBsAg-S Gene. Proceedings of the International Conference on Biology, Sciences and Education (ICoBioSE 2019). DOI https://doi.org/10.2991/absr.k.200807.067

BOOK CHAPTER

2021

Sandy Vantika, Udjianna S. Pasaribu, Sapto W. Indratno, Adi Pancoro. (2021). Estimating Corn Weight Using Mixed Model with Linear Covariance Function Matrix. In: E. Joelianto, A. Turnip, A. Widyotriatmo (eds) Cyber Physical, Computer and Automation System. Advances in Intelligent Systems and Computing, Vol 1291. Springer, Singapore. https://doi.org/10.1007/978-981-33-4062-6_15

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