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Ernawati Arifin Giri-Rachman, Ph.D

School of Life Sciences and Technology
Institut Teknologi Bandung
Indonesia
Jl. Ganesa 10 Bandung

+62-22-2511575/ +62-22-2534107
E-mail :erna@sith.itb.ac.id , ernagirirach@gmail.com

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[et_pb_accordion_item title=” Position”] Assistant Professor in Molecular Biology [/et_pb_accordion_item][et_pb_accordion_item title=” Academic career”]

Assistant Professor in Molecular Biology	Institut Teknologi Bandung	2006
Doctorate (Molecular Microbiology)	University of Bristol	2003
Master (Chemistry)	Institut Teknologi Bandung	1997
Bachelor (Chemistry)	Institut Teknologi Bandung	1993

[/et_pb_accordion_item][et_pb_accordion_item title=” Employment”] Academic staff (2006 – now) [/et_pb_accordion_item][et_pb_accordion_item title=” Research and development projects”]

1. Rispro LPDP, 2016, Produksi HBsAg dan Anti-HBsAg Untuk Pengembangan serta Komersialisasi Kit Diagnostik Hepatitis B Indonesia (Principal Investigator)
2. Riset Kerjasama Luar Negeri, 2017, Pengembangan Vaksin Hepatitis B Intranasal menggunakan P-Particle, kerjasama dengan Virginia Tech, US (Principal Investigator)
3. National Vaccine Consortium in Hepatitis B Insentif Riset Sinas-Kementrian Riset-Teknologi, 2012-…… (Co-Researcher, ITB Coordinator) Development and production of Subunit Hepatitis B Vaccine in Indonesia
4. Riset Centre and Advance Science ITB. 2015. Purification of P-particle and development of intranasal therapeutic and preventive Hepatitis B vaccine
5. Riset Inovasi KK ITB.2015. Studi dimerasisasi PhoR M.tuberculosis untuk pengembangan obat baru.
6. Riset Unggulan ITB.2015. Pengembangan humanized antibodi monoclonal anti-HBsAg
7. Riset Inovasi KK ITB.2014. Determination of 3-D conformation of Phor domain sensor of Mycobacterium tuberculosis for development of new anti tuberkular. 2014. Funded by Institut Teknologi Bandung
8. Cloning and crystallization of PhoR cytoplasmic domain from Mycobacterium tuberculosis to develop novel multitarget antitubercular drug. 2013. TWAS Grant
9. Pathogenesis and immunology of enteric virus. 2012. Fulbright Visiting Scholar 2012 : in Dr.Lijuan Yuan Laboratory, Virginia Tech, US.
10. Riset dan Inovasi KK ITB, 2012 (Princial Investigator) Production of Modified L-HBsAg in Plant as a Protective Hepatitis B Vaccine Antigen
11. Riset PP Pangan, Kesehatan dan Obat ITB, 2011 (Principle Investigator) Initiation of Hepatitis B Surface Antigen (HBsAg) Production based on Indonesian isolate in Pichia pastoris
12. Riset Hibah Kompetisi Strategis Nasional, DIKTI, 2009-2011 (Principle Investigator) Production of L-HBsAg in plant to solve National Health Problem
13. Riset Unggulan ITB, 2009 (Principle Investigator) Production of Hepatitis B surface antigen (HBsAg) and mutein G145R HBsAg in Pichia pastoris
14. Riset kompetitif LIPI- 2007-2011 (Co-Researcher) Development of Hepatitis B edible vaccine
15. Riset Desentralisasi DIKTI, 2012 (Principal Investigator) Purification and Crystalization of PhoR Sensor Domain Mycobacterium tuberculosis as a target of Novel Antitubercular
16. National Vaccine Consortium in Tuberkulosis, Insentif Riset Sinas-Kementrian Riset-Teknologi, 2012 (Co-Researcher, ITB Coordinator) Development of Prototype of Tuberculosis Vaccine
17. Indonesian Torray Science Foundation, 2009 (Co-researcher) Targetting PhoP-PhoR two component signal transduction to develop a novel high throughput screening system for searching new antibacterial against M . tuberculosis
18. Riset Internasional ITB, 2008, collaboration with School of Pharmacy ITB and University of Groningen, Netherlands (Co-Researcher) Molecular characterization of streptococcal adhesin to human fibronectin using Surface Plasmon Resonance
19. KNAW Mobility Program, collaboration with School of Pharmacy ITB and University of Groningen, Netherlands (Co-Researcher) Drugs development of Streptococcus Pyogenes
20. Joint Research Program Hibah Kompetisi B (PHK-B)-DIKTI, Kerjasama SITH-ITB dan Fakultas Kedokteran UK Maranatha : 2009 (Co-Researcher) Development of FCGamma RIII detection
21. Riset Hibah Kompetitif Strategis Nasional DIKTI, 2009 (Co-Researcher) Development of HCV diagnostic kit
22. Riset Insentif Kementrian Ristek dan Teknologi, 2007 (Co-Researcher) Microsateliate in Penaues Monodon
23. Riset Pembinaan Ilmu Kedokteran (Risbin-Iptekdok), Kerjasama dengan Universitas Hasanudin , 2007 (Konsultan) Overexpression of Cpn-1 as Tuberculosis vaccine candidate
24. Hibah Pekerti DIKTI, Kerjasama dengan Universitas Hasanudin , 2006 (Co-Researcher) VP 28 as White Spot Syndrome Virus vaccine candidate in shrimp
25. Riset Fakultas – ITB Kerjasama dengan Kelompok Keahlian Biokimia – ITB , 2006 (Coresearcher) Protein analysis of gene expressed in invation process of Salmonella typhi straind Indonesia
26. Riset Pembinaan Ilmu Kedokteran (Risbin-Iptekdok), Kerjasama dengan Universitas Hasanudin, 2006 (Consultant) Immune response variant against Anti-ESAT6 in M akasar
27. Intitation of collaboration with Dr. Claire Leuseur, National University of Singapore , 2005 Site directed mutagenesis of Cholera toxin B subunit to investigate cholera toxin assembly in vitro
28. Indonesian Torray Science Foundation, 2004 ( (Principal investigator) Study of D51 mutant of two component regulatory system of Vibrio cholerae
29. PhD Thesis, 2003 The Cpx two component regulatory system of Vibrio cholerae (Supervisor : Prof.T.R.Hirst, University of Bristol, UK)

[/et_pb_accordion_item][et_pb_accordion_item title=”Collaboration”]

PT. Biofarma (Persero) :

1. 2012 – now : National Vaccine Consortium in Hepatitis B
2. Rispro LPDP : 2016 – …
3. Program Immersi Ristek DIKTI 2016

[/et_pb_accordion_item][et_pb_accordion_item title=” Publications”]

Contribution to GenBank NCBI Database :

1. Efendi,Y.S., Susanti,D., Tritama,E., Putri,G.N.N., Raharso,S.,Iskandar,I., Aditiawati,P., Giri-Rachman,E.A., Mukhopadhyay,B. and Purwantini,E. Bordetella pertussis strain Pelita III, complete genome. GenBank : NZ_CP019957. 2017
2. Giri-Rachman,E.A ., Kocher,J.F., Li,G., Bui,T., Jiang,X. and Yuan,L. Norovirus Hu/GII.4 2006b/092895/2008/USA major capsid protein gene, complete cds.GenBank: KC990829.1. 2013
3. Jinata,C., Giri-Rachman,E.A . and Retnoningrum,D.S. Hepatitis B virus isolate CJ66 small S protein (sHBsAg) gene, partial cds. JQ646056.1-JQ646072, 2012 (18 sequences)

Journal :

1. Yusuf Efendi, Dwi Susanti, Erman Tritama, Michelle Pasier, Gilang Putri, Sugeng Raharso, Iskandar Iskandar, Pingkan Aditiawati, Ernawati Giri-Rachman, Biswarup Mukhopadhyay, and Endang Purwantini. Complete genome sequence of Bordetella pertussis Pelita III, the production strain for an Indonesian Whole Cell Pertussis Vaccine. Accepted in Genome Announcements.
2. Latri Rahmah, Ernawati G Rachman, Debbie S. Retnonigrum, Marselina I. Tan. 2017. Molecular characterization and expression of human Rotavirus Recombinant Protein VP2, VP6 and VP7 transfected in Vero Cell. International Journal of Integrative Biology. 18:1
3. Lei S, Ramesh A, Twitchell E, Wen K, Bui T, Weiss M, Yang X, Kocher J, Li G, Giri-Rachman E, Trang NV, Jiang X, Ryan EP, Yuan L. 2016. High Protective Efficacy of Probiotics and Rice Bran against Human Norovirus Infection and Diarrhea in Gnotobiotic Pigs. Front Microbiol. 2016, 7:1699
4. Wang H, Gao K, Wen K, Allen IC, Li G, Zhang W, Kocher J, Yang X, Giri-Rachman E, Li GH, Clark-Deener S, Yuan L. 2016. Lactobacillus rhamnosus GG modulates innate signaling pathway and cytokine responses to rotavirus vaccine in intestinal mononuclear cells of gnotobiotic pigs transplanted with human gut microbiota. BMC Microbiol. 16(1):109. doi: 10.1186/s12866-016-0727-2
5. Ernawati Arifin Giri-Rachman, Indah Woro Utami, Shinta Kusumawardani, Debbie Soefie Retnoningrum, Dessy Natalia, Nurfitriani, Gilang Nadia, Patricia Gita Naully and Neni Nurainy. 2015. Construction, Expression and Characterization of Multi Cassettes Encoding Indonesian Small Hepatitis B Surface Antigen (s-HBsAg) in Methylotropic Yeast Pichia pastoris. Biotechnology 14(5): 225-232 DOI: 10.3923/biotech.2015.225.232
6. Zhang H, Wang H, Shepherd M, Wen K, Li G, Yang X, Kocher J, Giri-Rachman E, Dickerman A, Settlage R, Yuan L. 2014. Probiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigs. Gut Pathog. 2014 Sep 9;6:39. doi: 10.1186/s13099-014-0039-8.
7. Oktira Roka Aji, Dyshelly Nurkartika Pascapurnama, Fenryco Pratama, Ihsanawati Ihsanawati, Maelita Ramdhani Moeis, Ernawati Arifin Giri-Rachman, 2014. Cloning, Overexpression, and Purification of PhoR CytoplasmicDomain Protein from Mycobacterium tuberculosis strain H37Rv. Vol 8, No 4 (2014)
8. Ernawati Arifin Giri-Rachman,Fenryco Pratama, Oktira Roka Aji, Arum Patriati, Ihsanawati, Maelita Ramdani Moeis, Edy Giri-Rachman Putra, Expressionand Purification of PhoR SensorDomain Histidine Kinaseof Mycobacterium tuberculosisin Eschericia coli. Submited to Microbiology Indonesia, under review
9. Jacob Kocher, Tammy Bui, Erna Giri-Rachman, Ke Wen, Guohua Li, Xingdong Yang,Fangning Liu, Ming Tan, Ming Xia, WeimingZhong, Xi Jiang, and Lijuan Yuan. 2014. Intranasal P particle vaccine protects against human GII.4 norovorus infection and diarrhea in gnotobiotic pigs. J Virol. pii: JVI.01249-14.
10. Ke Wen, Christine Tin, Haifeng Wang, Xingdong Yang,Guohua Li, Ernawati Giri-Rachman , Jacob Kocher, Tammy Bui, Sherrie Clark-Deener, Lijuan Yuan. 2014.Probiotic Lactobacillus rhamnosusGG enhanced Th1 cellular immunity but did not affect antibody responses in a human gut microbiota transplanted neonatal gnotobiotic pig model. PLoS One. 10;9(4):e94504. doi: 10.1371 Husen Zhang, Haifeng Wang, Megan Shepherd, Ke Wen, Guohua Li, Xingdong Yang, Jacob Kocher,Ernawati Giri-Rachman , Allan Dickerman, Robert Settlage, Lijuan Yuan. Lactobacillus rhamnosusGG modulates the human gut microbiota in rotavirus vaccinated and challenged gnotobiotic pigs. 2013. Submitted
11. Ugiyadi M, Tan MI, Giri-Rachman EA, Zuhairi FR, Sumarsono SH. The expression of essential components for human influenza virus internalisation in Vero and MDCK cells. Cytotechnology . Published online 2013 Aug 4
12. Martius E., Mardjuki A, Giri-Rachman, E , Riani C, Cahyati , Retnoningrum DS. 2013. Role of Lys59 and Lys386 of streptokinase from type 12 group A Streptococcus on in vitro stability against plasmin cleavage. Journal of Pure and Applied Microbiology , 7: 2617-2627
13. Chandra Jinata,Ernawati Arifin Giri-Rachman and Debbie Soefie Retnoningrum. 2012. Molecular Analysis of Immune Escape Mutants of Hepatitis B Virus from Local Clinical Samples. Microbiology Indonesia. 6 (1), 9-14.
14. FX Reymond Sutandy, Putri Dwi Utari, Tati Kristianti, Sony Suhandono, Amy Estiati and Ernawati A. Giri Rachman. 2012. Transformation and Analysis of L-HBsAg DNA Transient Expression in Tobacco (Nicotiana tabacum Linn.) Seedlings.Journal of Life Sciences and Technology (STM online Journal) School of Life Science and Technology, ITB, 1 (1) : 19-24
15. Zrimi, J., A. N. Ling, A. Giri-Rachman, G. Feverati, C. Lesieur., 2010. Cholera Toxin B Subunits Assemble into Pentamers – Proposition of a Fly-Casting Mechanism. Plos One 5 (12) e15347 (eISSN-1932-6203)
16. Aldina S. Suwanto, Ihsanawati and Ernawati Arifin Giri-Rachman. 2011. Molecular Cloning of PhoR Sensor Domain from Mycobacterium tuberculosis for Structure-Based Discovery of Novel AntiTubercular Drugs . Journal of Life Sciences . 5:454-459
17. Sony Suhandono, Rasi Fitria, Ernawati Arifin Giri Rachman . Sequence Analysis of PutativepotB , potC , and potD Genes from Serratia rubidae . Microbiology Indonesia . 4:2, (ISSN 1978-3477)
18. Patrisia Puspapriyanti, Johan Lucianus, Ernawati A. Giri-Rachman . 2010. Kloning DNA Pengkode PhoQ untuk Pengembangan High Throughput Screening System sebagai Usaha Mencari Antibakteri Baru terhadap Salmonella typhi. Jurnal Kedokteran Maranatha . 9(2): 136-145 (ISSN 1411-9641)
19. Kirby Saputra, Johan Lucianus, Ernawati A. Giri-Rachman. Deteksi mutasi pada Quinolone Resistance Determining Region (QRDRs) gen gyrA pada Salmonella typhi Isolat Klinik dan Galur khas Indonesia. Jurnal Kedokteran Maranatha 9(1): 1-7 (ISSN 1411-9641)
20. Suhandono S., Giri-Rachman E. A. , I.M Zainuddin, P D Utari, A. Supraba and A. Estiati. 2007. Construction of Binary Vector with Wound Inducible Promoter For HBsAg Expression : Development of Plant–based Edible Hepatitis B Vaccine from Indonesian isolate. Annales Bogorienses 11 (1): 8-18 (ISSN : 0517-8452).

Paten :

1. Terdaftar dan Dipublikasi:PLASMID REKOMBINAN YANG BERFUNGSI SEBAGAI VEKTOR EKSPRESI UNTUK PRODUKSI BERLEBIH HEPATITIS B SURFACE ANTIGEN (HBsAg) DI HANSENULA POLYMORPHA. No Pendaftaran : P00201407051. 2014. Paten diajukan bersama  PT Biofarma (Persero) dan ITB.Biofarma : Neni Nurainy, Gilang Nadia, Vanny NaritaITB : Dessy Natalia, Ernawati A. Giri-Rachman (SITH/PP Biosains Bioteknologi ITB), Debbie Soefie Retnoningrum dan Fernita Puspasari
2. Terdaftar :VIRUS–LIKE PARTICLE PROTEIN SMALL HEPATITIS b SURFACE ANTIGEN (sHBsAg) UNTUK BAHAN BAKU VAKSIN HEPATITIS B DAN PROSES PRODUKSINYA DI SEL INANG Pichia pastoris.No Pendaftaran : p00201504908. 12 Agustus 2015Paten diajukan bersama  PT Biofarma (Persero) dan ITBBiofarma : Neni Nurainy, Gilang Nadia, Dicky MahardhikaITB : Ernawati A. Giri-Rachman (SITH/PP Biosains Bioteknologi ITB), Dessy Natalia, Debbie Soefie Retnoningrum, Elvi Restiawaty, Wardono Niloperbowo dan Fernita Puspasari

Book :

Marselina Irasonia Tan, Anggraini Barlian, Ernawati A. Giri-Rachman, Biologi Sel dan Terapannya. Penerbit ITB. 2015

Proceeding :

1. Langi, Gladys Emmanuella Putri ; Moeis, Maelita R. ; Ihsanawati, Giri-Rachman, Ernawati Arifin , Molecular cloning and cold shock induced overexpression of the DNA encoding phor sensor domain from Mycobacterium tuberculosis as a target molecule for novel antitubercular drugs, AIP Conference Proceedings, Volume 1589, Issue 1, p.407-410

2. Dina Rachmi Ramdhani, Putri Dwi Utari, Ima Mulyama Zainuddin, Ami Estiati, Sony Suhandono,Ernawati A. Giri-Rachman. 2009. Construct DNA Encoded Large Hepatitis B Surface Antigens (L-HBsAg) from Indonesian Isolates into Expression of Vector pCAMBIA 1390 to Development of The Third Generation Vaccine. Proceeding of International Conference and Exhibition on Biomass Production , School of Life Sciences and Technology, ITB. ISBN 978-60296488-0-5

3. Ima Mulyama Zainuddin , Sony Suhandono, Ernawati Arifin Giri-Rachman , Ami Estiyati . 2007. “Construction of Binary Vector with MeEF1 Promoter for Gene Expression of Hepatitis B SurfaceAntigen (HBsAg) in Plant.” Proceeding in International Seminar on Pharmaceutics. School of Pharmacy Institut Teknologi Bandung.
4. Saripah Sundari, Junessa Palao, and Ernawati Giri-Rachman . Targetting PhoP-PhoR two component signal transduction to develop a novel high throughput screening system for searching new antibacterial against M . tuberculosis. Proceeding of International Conference and Exhibition on Biomass Production , School of Life Sciences and Technology, ITB . ISBN 978-602-96488-0-5
5. Mufti Hatur Rahmah and Ernawati A. Giri-Rachman . 2009. Amplification of truncated MprB encoding gene to Develop a Novel High ThroughputScreening System for Searching New Antibacterial against Mycobacterium Tuberculosis. Proceeding of International Conference and Exhibition on Biomass Production, School of Life Sciences and Technology . ISBN 978-602-96488-0-5

6. Ima Mulyama Zainuddin, Ima, Sony Suhandono, Ernawati A. Giri-Rachman , Amy Estiati.2008. Isolation of Poly-A vspB Fragment from Soybean cv. Slamet, Proceeding in International Conference of Mathematical and Natural Sciences

7. Ernawati Arifin Giri-Rachman , Thomas Robertus and Sarwono Hadi. 2006. “PCR for detectionSalmonella typhi strain Ind onesia.” Proceeding in International Conference of Mathematical and Natural Sciences.

Award :

I. Penghargaan Institut Teknologi Bandung Bidang Penelitian 2016

II. FULBRIGHT SENIOR RESEARCH PROGRAM , 2012

III. Poster/Oral Presentation :

1. Ernawati Arifin Giri-Rachman, Indah Woro Utami, Shinta Kusumawardani, Debbie Soefie Retnoningrum, Dessy Natalia, Nurfitrian,  Patricia Gita Naully, Neni Nurainy, Construction, Expression and Characterization of Multi Copy Expression Cassete of HBsAg  in Pichia pastoris. 7-8Mei 2015. International Seminar : Infectious Diseases, Biochemistry and Structural Biology.Molecular and Cellular Life Science (MCLS) :  Surabaya. The Best Presenter in Infectious Disease

2. Utari, P.D. , Suhandono. S., Ramadhani, D.R., Sutandy, FX.R., Badriyah, L., Kristianti, T., Estiati, A., dan Giri Rachman, E.A ., Construction and Transient Expression of Recombinant Large Hepatitis B Surface Antigen in Plants to Develop Third Generation Vaccine. Proceeding of The 5th International Eijkman Conference on the Eijkman Institute comes of age: vitamins, genomics, and welfare , 8-10 November 2011 . Best Oral Presentation
3. Dina Rachmi Ramdhani , Putri Dwi Utari, Ima Mulyama Zainuddin, Ami Estiati, Sony Suhandono,Ernawati A. Giri-Rachman. Construct DNA Encoded Large Hepatitis B Surface Antigens (L-HBsAg) from Indonesian Isolates into Expression of Vector pCAMBIA 1390 to Development of The Third Generation Vaccine. International Conference and Exhibition on Biomass Production, School of Life Sciences and Technology, ITB, November, 2009 . 2 nd Best Poster Presentation

4. Caroline T. Sardjono, Teresa L. Wargasetia, Ernawati G. Rachman , Marselina I. Tan. DETECTION OF FC GAMMA RECEPTOR III EXPRESSION IN HEMATOPOETIC STEM CELL, International Seminar and Workshop, 8th Basic Molecular Biology Course on Stem Cell, Treatment With Stem Cell. Brawijaya University Malang. May 2010, Best Poster Presentation

5. Junessa Palao, Saripah Sundari and Ernawati A.Giri-Rachman , Cloning of Promoter iclR coli K-12 in Vector pRSET with eGFP probe : Application Promoter iclR to develop Screening System for Searching New Antibiotic Against MDR-TB (Multi Drugs Resistant Tuberculosis), Kongres Nasinal VII PAMKI Pengendalian Penyakit Infeksi untuk meningkatkan kualitas hidup bangsa, 12 – 14 November 2009. Best poster presentation

6. Saripah Sundari, Junessa Palao, and Ernawati Giri-Rachman , Targetting PhoP-PhoR two component signal transduction to develop a novel high throughput screening system for searching new antibacterial against M . tuberculosis. Presentasi oral pada “Biotechnology for a Sustainable Future” Universitas Udayana, Bali, 15-16 September 2009. 2nd Best Oral Present ation
7. Kirby Saputra , Johan Lucianus, Ernawati A. Giri-Rachman. Deteksi mutasi pada Quinolone Resistance Determining Region (QRDRs) gen gyrA pada Salmonella typhi Isolat Klinik dan Galur khas Indonesia. Seminar Fakultas Kedokteran Universitas Kristen Maranatha, July 2009. Best poster presention
8. Mohammad Bahrelfi Belafiff, Ernawati A. Giri-Rachman , Adi Pancoro. Phylogeography and distribution pattern of H5N1 from Indonesia, the Second International Conference on Mathematics and Natural Sciences (ICMNS). Institut Teknologi Bandung. Best Oral Presentation . October 2008

Invited Speaker :

1. Exploring and Exploiting Indonesian Hepatitis B Virus for Generating Independence Vaccine. Ernawati A. Giri-Rachman and Neni Nurainy. ICMNS ITB 2016.
2. Virus and Virus Like Particle. FGD Bio-Nanomaterial untuk Aplikasi Kesehatan IPB-BATAN- ITB. Serpong, 12-13 Maret 2015
3. Cloning dengan Vektor pAO815, Transformasi di pastoris dan Purifikasi Protein Rekombinan. Workshop Vaksinologi Tahap III (penyeragaman metode). PT Biofarma, 1-2 September 2015
4. Virus dan Virus Like Particle. Kuliah Tamu Institut Teknologi Dell. Medan, 24 September 2015
5. Purification and Characterization of PhoR Mycobacterium tuberculosis as a Target for Structure Based Discovery of Novel Anti Tubercular Drugs. 56th International Annual Conference of The Association of Microbiologists of India.New Delhi, 7-10 Desember 2015

II. FULBRIGHT SENIOR RESEARCH PROGRAM , 2012

Conference (last 5 years) :

1. Ernawati Giri Rachman, Meliawati Poniman, Tina Lusiany, Stephanie Lie, Debbie Soefie Retnoningrum and Lijuan Yuan^. Cloning and Purification of Norovirus P Particle and In Silico Study as an Intranasal Hepatitis B Vaccine Candidate. ASV Meeting, Blacksburg US, 2016.
2. Rahmat Azhari Kemal, Siti Isnaeni Muthmainah, Vanny Narita, Ernawati Arifin Giri-Rachman, Dessy Natalia, Debbie Soefie Retnoningrum, Neni Nurainy. Construction and Expression of Multi Cassettes Encoding Indonesian Middle Hepatitis B Surface Antigen (M-HBsAg) in Pichia pastori ICMNS 2016.
3. Ika Agus Rini, Debbie S. Retnoningrum, Ernawati Giri Rachman, Neni Nurainy. Construction and Overexpression Optimization of HBcAg Protein With Vector pET-16b in Escherichia Coli BL21 (DE3) as a Therapeutic Vaccine Component. ICMNS 2016.
4. Ernawati Arifin Giri-Rachman, Indah Woro Utami, Shinta Kusumawardani, Debbie Soefie Retnoningrum, Dessy Natalia, Nurfitriani, Patricia Gita Naully, Neni Nurainy, Construction and Expression of Multi Cassettes Encoding Indonesian Small Hepatitis B Surface Antigen (s-HBsAg) in Methylotrophic Yeast Pichia pastoris. 1^st Asean Microbial Biotechnology Conference. 2014. Thailand
5. Febiana Rima Diputri, Ernawati A. Giri-Rachman, Konstruksi dan Overekspresi Fusi Terlarut Maltose Binding Protein (MBP) dan Domain Sitoplasmik Protein PhoR Mycobacterium tuberculosis sebagai Pengembangan Target Anti Tuberkulosis Baru. PIT PERMI 8-9 Oktober. 2015. Semarang
6. Meliawati Poniman, Ernawati A. Giri-Rachman, Stephanie Lie, Debbie Soefie Retnoningrum, Lijuan Yuan. Cloning and Purification of Norovirus P Particle and in silico Study as an Intranasal Hepaititis B Vaccine Candidate. PIT PERMI 8-9 Oktober. 2015. Semarang
7. Ricky Mandala Putra, Irma Sopiyani Kurnia dan Ernawati Arifin Giri-Rachman. Purifikasi Protein Domain Sitoplasmik PhoR Mycobacterium tuberculosis H37Rv untuk penapisan Antituberkulosis Baru. PIT PERMI 8-9 Oktober. 2015. Semarang
8. Shely Agustina, Vanny Narita, Ernawati Arifin Giri-Rachman, Siti Isnaeni Mutmainah, Dessy Natalia, Debbie Soefie Retnoningrum, Neni Nurainy. Clonind and Expression lf L-HBsAg Gene in Pichia pastoris GS 115. PIT PERMI 8-9 Oktober. 2015. Semarang
9. Mellysa Rahmita dan Ernawati A. Giri-Rachman. Pengembanga Sistem Penapisan Obat Antituberkulosis Baru dengan Target PhoR Mycobacterium tuberculosis H37 Rv Menggunakan Protein Repressor araC Escherichia coli. PIT PERMI 8-9 Oktober. 2015. Semarang
10. Nur Amalia Rosyada. dan Ernawati A. Giri-Rachman. Konstruksi dan Overekspresi Fusi Maltose Binding Protein dan Domain Sensor Protein PhoR Mycobacterium tuberculosis. PIT PERMI 8-9 Oktober. 2015. Semarang
11. Prima Nanda Fauziah, Sony Suhandono, Ernawati A. Giri-Rachman. Molecular Cloning, Characterization, Promoter Analysis of the Zinc Finger 23 (ZIF23) Gene to Deveop a Novel High Throughput Screening System or New Antitubercular by Targetting PhoR of Mycobacterium tuberculosis. PIT PERMI 8-9 Oktober. 2015. Semarang
12. Risma Wiharyanti, Made Tri Ari Penia Kresnowati, Ernawati Arifin Giri-Rachman , ISOLATION And CLONING OF PROMOTER FORMATE DEHYDROGENASE (FDH) FROM Candida orthopsilosi. INTERNATIONAL SEMINAR AND MINI WORKSHOP ON PHARMACEUTICS. School of Pharmacy ITB. Advanced Research in Pharmaceutical Science and Technology. 2015
13. Maharani Ugayadi, Marselina I Tan,Ernawati A Giri-Rahman , Fawzi R Zuhairi, Sony H Sumarsono. Primary monkey trachea cell culture express the subtantial components for influenza virus internalization. International Seminar on Tropical Bioresources for Sustainable Bioindustry. ITB Bandung, 30–31 October 2012
14. Regina Angeline, Rahma Tejawati Maryama,Ernawati Arifin Giri-Rachman and Wardono Niloperbowo. Mathematical Modelling of Growth of Recombinant Pichia pastoris , Producing sHBsAg, in Yeast Extract Potato Dextrose Medium (YEPD) and Buffered Glycerol-complex Medium (BMGY). International Conference on Mathematical and Natural Science . Institut Teknologi Bandung. 8-9 November 2012. Oral Presentation
15. Amy Estiati, Ade Nena,Ernawati A. Giri-Rachman , Putri Sandy Pangestu, Putri Dwi Utari, Ima Mulyama and Sony Suhandono. Transformation of Hepatitis B Surface Antigen into Local Banana Plants for Molecular Farming Vaccine. International Conference on Biomedical Science. Oral Poster Presentation. School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation.
16. Rahma Tejawati Maryama, Shinta Kusumawardhani, Dessy Natalia, Debbie S Retnoningrum andErnawati A. Giri-Rachman . Intracelullar Expression of HbsAg Genotype B in Pichia pastoris KM71 Hyperresistant Zeosin, International Conference on Biomedical Science . School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation.
17. Putri Dwi Utari, Dina Rachmi Ramadhani, FX Reymond Sutandy, Lailatul Badriyah, Tati Kristanti, Ami Estiati, Sony Suhandono andErnawati Arifin Giri-Rachman . Construction and Transient Expression of Recombinant Large Hepatitis B Surface Antigen (L-HBsAg) in Plants to Develop Third Generation Hepatitis B Vaccine. International Conference on Biomedical Science . School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation.

[/et_pb_accordion_item][et_pb_accordion_item title=” Lectures (last 5 years)”]

1. Basic Molecular and Cell Biology (For Microbiology Study Program)
2. Applied in Molecular and Cell Biology (For School of Pharmacy)
3. Diagnostic Microbiology
4. Microbial physiolgy (practical)
5. Microbial pathogenesis and Immunology
6. Project on Microbial Genetics
7. Virology
8. Current Issues in Biotechnology

[/et_pb_accordion_item][et_pb_accordion_item title=”Professional Organization”]

1. Society of Indonesian Microbiology, Bandung Branch Chairman Juni 2011

2. Society of Indonesian Microbiology, Bandung Branch Program Coordinator 2005-2011

3. Society of Indonesian Biochemistry and Molecular Biology, Bandung Branch Member 2011

[/et_pb_accordion_item][et_pb_accordion_item title=” Reviewer”]

Indonesian Torray Science Foundation Research Grant, 2006, 2008

[/et_pb_accordion_item][et_pb_accordion_item title=” Editor”]

Editorial Team : Microbiology Indonesia 2012

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[/et_pb_accordion][/et_pb_column][/et_pb_row][/et_pb_section][:id][et_pb_section bb_built=”1″ fullwidth=”on” specialty=”off”][et_pb_fullwidth_menu _builder_version=”3.0.63″ menu_id=”3″ background_color=”#54a275″ submenu_direction=”downwards” fullwidth_menu=”off” background_layout=”light” text_orientation=”left” dropdown_menu_animation=”fade” /][/et_pb_section][et_pb_section bb_built=”1″ fullwidth=”off” specialty=”off”][et_pb_row][et_pb_column type=”1_4″][et_pb_image _builder_version=”3.0.63″ src=”https://multisite.itb.ac.id/sith/wp-content/uploads/sites/56/2017/02/erna.jpg” show_in_lightbox=”off” url_new_window=”off” use_overlay=”off” sticky=”off” align=”left” always_center_on_mobile=”on” border_style=”solid” force_fullwidth=”off” /][/et_pb_column][et_pb_column type=”3_4″][et_pb_text _builder_version=”3.0.63″ background_layout=”light” text_orientation=”left” border_style=”solid”]

Ernawati Arifin Giri-Rachman, Ph.D

School of Life Sciences and Technology
Institut Teknologi Bandung
Indonesia
Jl. Ganesa 10 Bandung

+62-22-2511575/ +62-22-2534107
E-mail :erna@sith.itb.ac.id , ernagirirach@gmail.com

[/et_pb_text][/et_pb_column][/et_pb_row][et_pb_row][et_pb_column type=”4_4″][et_pb_accordion _builder_version=”3.0.63″ border_style=”solid”] [et_pb_accordion_item title=” Position”] Assistant Professor in Molecular Biology [/et_pb_accordion_item][et_pb_accordion_item title=” Academic career”]

Assistant Professor in Molecular Biology	Institut Teknologi Bandung	2006
Doctorate (Molecular Microbiology)	University of Bristol	2003
Master (Chemistry)	Institut Teknologi Bandung	1997
Bachelor (Chemistry)	Institut Teknologi Bandung	1993

[/et_pb_accordion_item][et_pb_accordion_item title=” Employment”] Academic staff (2006 – now) [/et_pb_accordion_item][et_pb_accordion_item title=” Research and development projects”]

1. Rispro LPDP, 2016, Produksi HBsAg dan Anti-HBsAg Untuk Pengembangan serta Komersialisasi Kit Diagnostik Hepatitis B Indonesia (Principal Investigator)
2. Riset Kerjasama Luar Negeri, 2017, Pengembangan Vaksin Hepatitis B Intranasal menggunakan P-Particle, kerjasama dengan Virginia Tech, US (Principal Investigator)
3. National Vaccine Consortium in Hepatitis B Insentif Riset Sinas-Kementrian Riset-Teknologi, 2012-…… (Co-Researcher, ITB Coordinator) Development and production of Subunit Hepatitis B Vaccine in Indonesia
4. Riset Centre and Advance Science ITB. 2015. Purification of P-particle and development of intranasal therapeutic and preventive Hepatitis B vaccine
5. Riset Inovasi KK ITB.2015. Studi dimerasisasi PhoR M.tuberculosis untuk pengembangan obat baru.
6. Riset Unggulan ITB.2015. Pengembangan humanized antibodi monoclonal anti-HBsAg
7. Riset Inovasi KK ITB.2014. Determination of 3-D conformation of Phor domain sensor of Mycobacterium tuberculosis for development of new anti tuberkular. 2014. Funded by Institut Teknologi Bandung
8. Cloning and crystallization of PhoR cytoplasmic domain from Mycobacterium tuberculosis to develop novel multitarget antitubercular drug. 2013. TWAS Grant
9. Pathogenesis and immunology of enteric virus. 2012. Fulbright Visiting Scholar 2012 : in Dr.Lijuan Yuan Laboratory, Virginia Tech, US.
10. Riset dan Inovasi KK ITB, 2012 (Princial Investigator) Production of Modified L-HBsAg in Plant as a Protective Hepatitis B Vaccine Antigen
11. Riset PP Pangan, Kesehatan dan Obat ITB, 2011 (Principle Investigator) Initiation of Hepatitis B Surface Antigen (HBsAg) Production based on Indonesian isolate in Pichia pastoris
12. Riset Hibah Kompetisi Strategis Nasional, DIKTI, 2009-2011 (Principle Investigator) Production of L-HBsAg in plant to solve National Health Problem
13. Riset Unggulan ITB, 2009 (Principle Investigator) Production of Hepatitis B surface antigen (HBsAg) and mutein G145R HBsAg in Pichia pastoris
14. Riset kompetitif LIPI- 2007-2011 (Co-Researcher) Development of Hepatitis B edible vaccine
15. Riset Desentralisasi DIKTI, 2012 (Principal Investigator) Purification and Crystalization of PhoR Sensor Domain Mycobacterium tuberculosis as a target of Novel Antitubercular
16. National Vaccine Consortium in Tuberkulosis, Insentif Riset Sinas-Kementrian Riset-Teknologi, 2012 (Co-Researcher, ITB Coordinator) Development of Prototype of Tuberculosis Vaccine
17. Indonesian Torray Science Foundation, 2009 (Co-researcher) Targetting PhoP-PhoR two component signal transduction to develop a novel high throughput screening system for searching new antibacterial against M . tuberculosis
18. Riset Internasional ITB, 2008, collaboration with School of Pharmacy ITB and University of Groningen, Netherlands (Co-Researcher) Molecular characterization of streptococcal adhesin to human fibronectin using Surface Plasmon Resonance
19. KNAW Mobility Program, collaboration with School of Pharmacy ITB and University of Groningen, Netherlands (Co-Researcher) Drugs development of Streptococcus Pyogenes
20. Joint Research Program Hibah Kompetisi B (PHK-B)-DIKTI, Kerjasama SITH-ITB dan Fakultas Kedokteran UK Maranatha : 2009 (Co-Researcher) Development of FCGamma RIII detection
21. Riset Hibah Kompetitif Strategis Nasional DIKTI, 2009 (Co-Researcher) Development of HCV diagnostic kit
22. Riset Insentif Kementrian Ristek dan Teknologi, 2007 (Co-Researcher) Microsateliate in Penaues Monodon
23. Riset Pembinaan Ilmu Kedokteran (Risbin-Iptekdok), Kerjasama dengan Universitas Hasanudin , 2007 (Konsultan) Overexpression of Cpn-1 as Tuberculosis vaccine candidate
24. Hibah Pekerti DIKTI, Kerjasama dengan Universitas Hasanudin , 2006 (Co-Researcher) VP 28 as White Spot Syndrome Virus vaccine candidate in shrimp
25. Riset Fakultas – ITB Kerjasama dengan Kelompok Keahlian Biokimia – ITB , 2006 (Coresearcher) Protein analysis of gene expressed in invation process of Salmonella typhi straind Indonesia
26. Riset Pembinaan Ilmu Kedokteran (Risbin-Iptekdok), Kerjasama dengan Universitas Hasanudin, 2006 (Consultant) Immune response variant against Anti-ESAT6 in M akasar
27. Intitation of collaboration with Dr. Claire Leuseur, National University of Singapore , 2005 Site directed mutagenesis of Cholera toxin B subunit to investigate cholera toxin assembly in vitro
28. Indonesian Torray Science Foundation, 2004 ( (Principal investigator) Study of D51 mutant of two component regulatory system of Vibrio cholerae
29. PhD Thesis, 2003 The Cpx two component regulatory system of Vibrio cholerae (Supervisor : Prof.T.R.Hirst, University of Bristol, UK)

[/et_pb_accordion_item][et_pb_accordion_item title=”Collaboration”]

PT. Biofarma (Persero) :

1. 2012 – now : National Vaccine Consortium in Hepatitis B
2. Rispro LPDP : 2016 – …
3. Program Immersi Ristek DIKTI 2016

[/et_pb_accordion_item][et_pb_accordion_item title=” Publications”]

Contribution to GenBank NCBI Database :

1. Efendi,Y.S., Susanti,D., Tritama,E., Putri,G.N.N., Raharso,S.,Iskandar,I., Aditiawati,P., Giri-Rachman,E.A., Mukhopadhyay,B. and Purwantini,E. Bordetella pertussis strain Pelita III, complete genome. GenBank : NZ_CP019957. 2017
2. Giri-Rachman,E.A ., Kocher,J.F., Li,G., Bui,T., Jiang,X. and Yuan,L. Norovirus Hu/GII.4 2006b/092895/2008/USA major capsid protein gene, complete cds.GenBank: KC990829.1. 2013
3. Jinata,C., Giri-Rachman,E.A . and Retnoningrum,D.S. Hepatitis B virus isolate CJ66 small S protein (sHBsAg) gene, partial cds. JQ646056.1-JQ646072, 2012 (18 sequences)

Journal :

1. Yusuf Efendi, Dwi Susanti, Erman Tritama, Michelle Pasier, Gilang Putri, Sugeng Raharso, Iskandar Iskandar, Pingkan Aditiawati, Ernawati Giri-Rachman, Biswarup Mukhopadhyay, and Endang Purwantini. Complete genome sequence of Bordetella pertussis Pelita III, the production strain for an Indonesian Whole Cell Pertussis Vaccine. Accepted in Genome Announcements.
2. Latri Rahmah, Ernawati G Rachman, Debbie S. Retnonigrum, Marselina I. Tan. 2017. Molecular characterization and expression of human Rotavirus Recombinant Protein VP2, VP6 and VP7 transfected in Vero Cell. International Journal of Integrative Biology. 18:1
3. Lei S, Ramesh A, Twitchell E, Wen K, Bui T, Weiss M, Yang X, Kocher J, Li G, Giri-Rachman E, Trang NV, Jiang X, Ryan EP, Yuan L. 2016. High Protective Efficacy of Probiotics and Rice Bran against Human Norovirus Infection and Diarrhea in Gnotobiotic Pigs. Front Microbiol. 2016, 7:1699
4. Wang H, Gao K, Wen K, Allen IC, Li G, Zhang W, Kocher J, Yang X, Giri-Rachman E, Li GH, Clark-Deener S, Yuan L. 2016. Lactobacillus rhamnosus GG modulates innate signaling pathway and cytokine responses to rotavirus vaccine in intestinal mononuclear cells of gnotobiotic pigs transplanted with human gut microbiota. BMC Microbiol. 16(1):109. doi: 10.1186/s12866-016-0727-2
5. Ernawati Arifin Giri-Rachman, Indah Woro Utami, Shinta Kusumawardani, Debbie Soefie Retnoningrum, Dessy Natalia, Nurfitriani, Gilang Nadia, Patricia Gita Naully and Neni Nurainy. 2015. Construction, Expression and Characterization of Multi Cassettes Encoding Indonesian Small Hepatitis B Surface Antigen (s-HBsAg) in Methylotropic Yeast Pichia pastoris. Biotechnology 14(5): 225-232 DOI: 10.3923/biotech.2015.225.232
6. Zhang H, Wang H, Shepherd M, Wen K, Li G, Yang X, Kocher J, Giri-Rachman E, Dickerman A, Settlage R, Yuan L. 2014. Probiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigs. Gut Pathog. 2014 Sep 9;6:39. doi: 10.1186/s13099-014-0039-8.
7. Oktira Roka Aji, Dyshelly Nurkartika Pascapurnama, Fenryco Pratama, Ihsanawati Ihsanawati, Maelita Ramdhani Moeis, Ernawati Arifin Giri-Rachman, 2014. Cloning, Overexpression, and Purification of PhoR CytoplasmicDomain Protein from Mycobacterium tuberculosis strain H37Rv. Vol 8, No 4 (2014)
8. Ernawati Arifin Giri-Rachman,Fenryco Pratama, Oktira Roka Aji, Arum Patriati, Ihsanawati, Maelita Ramdani Moeis, Edy Giri-Rachman Putra, Expressionand Purification of PhoR SensorDomain Histidine Kinaseof Mycobacterium tuberculosisin Eschericia coli. Submited to Microbiology Indonesia, under review
9. Jacob Kocher, Tammy Bui, Erna Giri-Rachman, Ke Wen, Guohua Li, Xingdong Yang,Fangning Liu, Ming Tan, Ming Xia, WeimingZhong, Xi Jiang, and Lijuan Yuan. 2014. Intranasal P particle vaccine protects against human GII.4 norovorus infection and diarrhea in gnotobiotic pigs. J Virol. pii: JVI.01249-14.
10. Ke Wen, Christine Tin, Haifeng Wang, Xingdong Yang,Guohua Li, Ernawati Giri-Rachman , Jacob Kocher, Tammy Bui, Sherrie Clark-Deener, Lijuan Yuan. 2014.Probiotic Lactobacillus rhamnosusGG enhanced Th1 cellular immunity but did not affect antibody responses in a human gut microbiota transplanted neonatal gnotobiotic pig model. PLoS One. 10;9(4):e94504. doi: 10.1371 Husen Zhang, Haifeng Wang, Megan Shepherd, Ke Wen, Guohua Li, Xingdong Yang, Jacob Kocher,Ernawati Giri-Rachman , Allan Dickerman, Robert Settlage, Lijuan Yuan. Lactobacillus rhamnosusGG modulates the human gut microbiota in rotavirus vaccinated and challenged gnotobiotic pigs. 2013. Submitted
11. Ugiyadi M, Tan MI, Giri-Rachman EA, Zuhairi FR, Sumarsono SH. The expression of essential components for human influenza virus internalisation in Vero and MDCK cells. Cytotechnology . Published online 2013 Aug 4
12. Martius E., Mardjuki A, Giri-Rachman, E , Riani C, Cahyati , Retnoningrum DS. 2013. Role of Lys59 and Lys386 of streptokinase from type 12 group A Streptococcus on in vitro stability against plasmin cleavage. Journal of Pure and Applied Microbiology , 7: 2617-2627
13. Chandra Jinata,Ernawati Arifin Giri-Rachman and Debbie Soefie Retnoningrum. 2012. Molecular Analysis of Immune Escape Mutants of Hepatitis B Virus from Local Clinical Samples. Microbiology Indonesia. 6 (1), 9-14.
14. FX Reymond Sutandy, Putri Dwi Utari, Tati Kristianti, Sony Suhandono, Amy Estiati and Ernawati A. Giri Rachman. 2012. Transformation and Analysis of L-HBsAg DNA Transient Expression in Tobacco (Nicotiana tabacum Linn.) Seedlings.Journal of Life Sciences and Technology (STM online Journal) School of Life Science and Technology, ITB, 1 (1) : 19-24
15. Zrimi, J., A. N. Ling, A. Giri-Rachman, G. Feverati, C. Lesieur., 2010. Cholera Toxin B Subunits Assemble into Pentamers – Proposition of a Fly-Casting Mechanism. Plos One 5 (12) e15347 (eISSN-1932-6203)
16. Aldina S. Suwanto, Ihsanawati and Ernawati Arifin Giri-Rachman. 2011. Molecular Cloning of PhoR Sensor Domain from Mycobacterium tuberculosis for Structure-Based Discovery of Novel AntiTubercular Drugs . Journal of Life Sciences . 5:454-459
17. Sony Suhandono, Rasi Fitria, Ernawati Arifin Giri Rachman . Sequence Analysis of PutativepotB , potC , and potD Genes from Serratia rubidae . Microbiology Indonesia . 4:2, (ISSN 1978-3477)
18. Patrisia Puspapriyanti, Johan Lucianus, Ernawati A. Giri-Rachman . 2010. Kloning DNA Pengkode PhoQ untuk Pengembangan High Throughput Screening System sebagai Usaha Mencari Antibakteri Baru terhadap Salmonella typhi. Jurnal Kedokteran Maranatha . 9(2): 136-145 (ISSN 1411-9641)
19. Kirby Saputra, Johan Lucianus, Ernawati A. Giri-Rachman. Deteksi mutasi pada Quinolone Resistance Determining Region (QRDRs) gen gyrA pada Salmonella typhi Isolat Klinik dan Galur khas Indonesia. Jurnal Kedokteran Maranatha 9(1): 1-7 (ISSN 1411-9641)
20. Suhandono S., Giri-Rachman E. A. , I.M Zainuddin, P D Utari, A. Supraba and A. Estiati. 2007. Construction of Binary Vector with Wound Inducible Promoter For HBsAg Expression : Development of Plant–based Edible Hepatitis B Vaccine from Indonesian isolate. Annales Bogorienses 11 (1): 8-18 (ISSN : 0517-8452).

Paten :

1. Terdaftar dan Dipublikasi:PLASMID REKOMBINAN YANG BERFUNGSI SEBAGAI VEKTOR EKSPRESI UNTUK PRODUKSI BERLEBIH HEPATITIS B SURFACE ANTIGEN (HBsAg) DI HANSENULA POLYMORPHA. No Pendaftaran : P00201407051. 2014. Paten diajukan bersama  PT Biofarma (Persero) dan ITB.Biofarma : Neni Nurainy, Gilang Nadia, Vanny NaritaITB : Dessy Natalia, Ernawati A. Giri-Rachman (SITH/PP Biosains Bioteknologi ITB), Debbie Soefie Retnoningrum dan Fernita Puspasari
2. Terdaftar :VIRUS–LIKE PARTICLE PROTEIN SMALL HEPATITIS b SURFACE ANTIGEN (sHBsAg) UNTUK BAHAN BAKU VAKSIN HEPATITIS B DAN PROSES PRODUKSINYA DI SEL INANG Pichia pastoris.No Pendaftaran : p00201504908. 12 Agustus 2015Paten diajukan bersama  PT Biofarma (Persero) dan ITBBiofarma : Neni Nurainy, Gilang Nadia, Dicky MahardhikaITB : Ernawati A. Giri-Rachman (SITH/PP Biosains Bioteknologi ITB), Dessy Natalia, Debbie Soefie Retnoningrum, Elvi Restiawaty, Wardono Niloperbowo dan Fernita Puspasari

Book :

Marselina Irasonia Tan, Anggraini Barlian, Ernawati A. Giri-Rachman, Biologi Sel dan Terapannya. Penerbit ITB. 2015

Proceeding :

1. Langi, Gladys Emmanuella Putri ; Moeis, Maelita R. ; Ihsanawati, Giri-Rachman, Ernawati Arifin , Molecular cloning and cold shock induced overexpression of the DNA encoding phor sensor domain from Mycobacterium tuberculosis as a target molecule for novel antitubercular drugs, AIP Conference Proceedings, Volume 1589, Issue 1, p.407-410

2. Dina Rachmi Ramdhani, Putri Dwi Utari, Ima Mulyama Zainuddin, Ami Estiati, Sony Suhandono,Ernawati A. Giri-Rachman. 2009. Construct DNA Encoded Large Hepatitis B Surface Antigens (L-HBsAg) from Indonesian Isolates into Expression of Vector pCAMBIA 1390 to Development of The Third Generation Vaccine. Proceeding of International Conference and Exhibition on Biomass Production , School of Life Sciences and Technology, ITB. ISBN 978-60296488-0-5

3. Ima Mulyama Zainuddin , Sony Suhandono, Ernawati Arifin Giri-Rachman , Ami Estiyati . 2007. “Construction of Binary Vector with MeEF1 Promoter for Gene Expression of Hepatitis B SurfaceAntigen (HBsAg) in Plant.” Proceeding in International Seminar on Pharmaceutics. School of Pharmacy Institut Teknologi Bandung.
4. Saripah Sundari, Junessa Palao, and Ernawati Giri-Rachman . Targetting PhoP-PhoR two component signal transduction to develop a novel high throughput screening system for searching new antibacterial against M . tuberculosis. Proceeding of International Conference and Exhibition on Biomass Production , School of Life Sciences and Technology, ITB . ISBN 978-602-96488-0-5
5. Mufti Hatur Rahmah and Ernawati A. Giri-Rachman . 2009. Amplification of truncated MprB encoding gene to Develop a Novel High ThroughputScreening System for Searching New Antibacterial against Mycobacterium Tuberculosis. Proceeding of International Conference and Exhibition on Biomass Production, School of Life Sciences and Technology . ISBN 978-602-96488-0-5

6. Ima Mulyama Zainuddin, Ima, Sony Suhandono, Ernawati A. Giri-Rachman , Amy Estiati.2008. Isolation of Poly-A vspB Fragment from Soybean cv. Slamet, Proceeding in International Conference of Mathematical and Natural Sciences

7. Ernawati Arifin Giri-Rachman , Thomas Robertus and Sarwono Hadi. 2006. “PCR for detectionSalmonella typhi strain Ind onesia.” Proceeding in International Conference of Mathematical and Natural Sciences.

Award :

I. Penghargaan Institut Teknologi Bandung Bidang Penelitian 2016

II. FULBRIGHT SENIOR RESEARCH PROGRAM , 2012

III. Poster/Oral Presentation :

1. Ernawati Arifin Giri-Rachman, Indah Woro Utami, Shinta Kusumawardani, Debbie Soefie Retnoningrum, Dessy Natalia, Nurfitrian,  Patricia Gita Naully, Neni Nurainy, Construction, Expression and Characterization of Multi Copy Expression Cassete of HBsAg  in Pichia pastoris. 7-8Mei 2015. International Seminar : Infectious Diseases, Biochemistry and Structural Biology.Molecular and Cellular Life Science (MCLS) :  Surabaya. The Best Presenter in Infectious Disease

2. Utari, P.D. , Suhandono. S., Ramadhani, D.R., Sutandy, FX.R., Badriyah, L., Kristianti, T., Estiati, A., dan Giri Rachman, E.A ., Construction and Transient Expression of Recombinant Large Hepatitis B Surface Antigen in Plants to Develop Third Generation Vaccine. Proceeding of The 5th International Eijkman Conference on the Eijkman Institute comes of age: vitamins, genomics, and welfare , 8-10 November 2011 . Best Oral Presentation
3. Dina Rachmi Ramdhani , Putri Dwi Utari, Ima Mulyama Zainuddin, Ami Estiati, Sony Suhandono,Ernawati A. Giri-Rachman. Construct DNA Encoded Large Hepatitis B Surface Antigens (L-HBsAg) from Indonesian Isolates into Expression of Vector pCAMBIA 1390 to Development of The Third Generation Vaccine. International Conference and Exhibition on Biomass Production, School of Life Sciences and Technology, ITB, November, 2009 . 2 nd Best Poster Presentation

4. Caroline T. Sardjono, Teresa L. Wargasetia, Ernawati G. Rachman , Marselina I. Tan. DETECTION OF FC GAMMA RECEPTOR III EXPRESSION IN HEMATOPOETIC STEM CELL, International Seminar and Workshop, 8th Basic Molecular Biology Course on Stem Cell, Treatment With Stem Cell. Brawijaya University Malang. May 2010, Best Poster Presentation

5. Junessa Palao, Saripah Sundari and Ernawati A.Giri-Rachman , Cloning of Promoter iclR coli K-12 in Vector pRSET with eGFP probe : Application Promoter iclR to develop Screening System for Searching New Antibiotic Against MDR-TB (Multi Drugs Resistant Tuberculosis), Kongres Nasinal VII PAMKI Pengendalian Penyakit Infeksi untuk meningkatkan kualitas hidup bangsa, 12 – 14 November 2009. Best poster presentation

6. Saripah Sundari, Junessa Palao, and Ernawati Giri-Rachman , Targetting PhoP-PhoR two component signal transduction to develop a novel high throughput screening system for searching new antibacterial against M . tuberculosis. Presentasi oral pada “Biotechnology for a Sustainable Future” Universitas Udayana, Bali, 15-16 September 2009. 2nd Best Oral Present ation
7. Kirby Saputra , Johan Lucianus, Ernawati A. Giri-Rachman. Deteksi mutasi pada Quinolone Resistance Determining Region (QRDRs) gen gyrA pada Salmonella typhi Isolat Klinik dan Galur khas Indonesia. Seminar Fakultas Kedokteran Universitas Kristen Maranatha, July 2009. Best poster presention
8. Mohammad Bahrelfi Belafiff, Ernawati A. Giri-Rachman , Adi Pancoro. Phylogeography and distribution pattern of H5N1 from Indonesia, the Second International Conference on Mathematics and Natural Sciences (ICMNS). Institut Teknologi Bandung. Best Oral Presentation . October 2008

Invited Speaker :

1. Exploring and Exploiting Indonesian Hepatitis B Virus for Generating Independence Vaccine. Ernawati A. Giri-Rachman and Neni Nurainy. ICMNS ITB 2016.
2. Virus and Virus Like Particle. FGD Bio-Nanomaterial untuk Aplikasi Kesehatan IPB-BATAN- ITB. Serpong, 12-13 Maret 2015
3. Cloning dengan Vektor pAO815, Transformasi di pastoris dan Purifikasi Protein Rekombinan. Workshop Vaksinologi Tahap III (penyeragaman metode). PT Biofarma, 1-2 September 2015
4. Virus dan Virus Like Particle. Kuliah Tamu Institut Teknologi Dell. Medan, 24 September 2015
5. Purification and Characterization of PhoR Mycobacterium tuberculosis as a Target for Structure Based Discovery of Novel Anti Tubercular Drugs. 56th International Annual Conference of The Association of Microbiologists of India.New Delhi, 7-10 Desember 2015

II. FULBRIGHT SENIOR RESEARCH PROGRAM , 2012

Conference (last 5 years) :

1. Ernawati Giri Rachman, Meliawati Poniman, Tina Lusiany, Stephanie Lie, Debbie Soefie Retnoningrum and Lijuan Yuan^. Cloning and Purification of Norovirus P Particle and In Silico Study as an Intranasal Hepatitis B Vaccine Candidate. ASV Meeting, Blacksburg US, 2016.
2. Rahmat Azhari Kemal, Siti Isnaeni Muthmainah, Vanny Narita, Ernawati Arifin Giri-Rachman, Dessy Natalia, Debbie Soefie Retnoningrum, Neni Nurainy. Construction and Expression of Multi Cassettes Encoding Indonesian Middle Hepatitis B Surface Antigen (M-HBsAg) in Pichia pastori ICMNS 2016.
3. Ika Agus Rini, Debbie S. Retnoningrum, Ernawati Giri Rachman, Neni Nurainy. Construction and Overexpression Optimization of HBcAg Protein With Vector pET-16b in Escherichia Coli BL21 (DE3) as a Therapeutic Vaccine Component. ICMNS 2016.
4. Ernawati Arifin Giri-Rachman, Indah Woro Utami, Shinta Kusumawardani, Debbie Soefie Retnoningrum, Dessy Natalia, Nurfitriani, Patricia Gita Naully, Neni Nurainy, Construction and Expression of Multi Cassettes Encoding Indonesian Small Hepatitis B Surface Antigen (s-HBsAg) in Methylotrophic Yeast Pichia pastoris. 1^st Asean Microbial Biotechnology Conference. 2014. Thailand
5. Febiana Rima Diputri, Ernawati A. Giri-Rachman, Konstruksi dan Overekspresi Fusi Terlarut Maltose Binding Protein (MBP) dan Domain Sitoplasmik Protein PhoR Mycobacterium tuberculosis sebagai Pengembangan Target Anti Tuberkulosis Baru. PIT PERMI 8-9 Oktober. 2015. Semarang
6. Meliawati Poniman, Ernawati A. Giri-Rachman, Stephanie Lie, Debbie Soefie Retnoningrum, Lijuan Yuan. Cloning and Purification of Norovirus P Particle and in silico Study as an Intranasal Hepaititis B Vaccine Candidate. PIT PERMI 8-9 Oktober. 2015. Semarang
7. Ricky Mandala Putra, Irma Sopiyani Kurnia dan Ernawati Arifin Giri-Rachman. Purifikasi Protein Domain Sitoplasmik PhoR Mycobacterium tuberculosis H37Rv untuk penapisan Antituberkulosis Baru. PIT PERMI 8-9 Oktober. 2015. Semarang
8. Shely Agustina, Vanny Narita, Ernawati Arifin Giri-Rachman, Siti Isnaeni Mutmainah, Dessy Natalia, Debbie Soefie Retnoningrum, Neni Nurainy. Clonind and Expression lf L-HBsAg Gene in Pichia pastoris GS 115. PIT PERMI 8-9 Oktober. 2015. Semarang
9. Mellysa Rahmita dan Ernawati A. Giri-Rachman. Pengembanga Sistem Penapisan Obat Antituberkulosis Baru dengan Target PhoR Mycobacterium tuberculosis H37 Rv Menggunakan Protein Repressor araC Escherichia coli. PIT PERMI 8-9 Oktober. 2015. Semarang
10. Nur Amalia Rosyada. dan Ernawati A. Giri-Rachman. Konstruksi dan Overekspresi Fusi Maltose Binding Protein dan Domain Sensor Protein PhoR Mycobacterium tuberculosis. PIT PERMI 8-9 Oktober. 2015. Semarang
11. Prima Nanda Fauziah, Sony Suhandono, Ernawati A. Giri-Rachman. Molecular Cloning, Characterization, Promoter Analysis of the Zinc Finger 23 (ZIF23) Gene to Deveop a Novel High Throughput Screening System or New Antitubercular by Targetting PhoR of Mycobacterium tuberculosis. PIT PERMI 8-9 Oktober. 2015. Semarang
12. Risma Wiharyanti, Made Tri Ari Penia Kresnowati, Ernawati Arifin Giri-Rachman , ISOLATION And CLONING OF PROMOTER FORMATE DEHYDROGENASE (FDH) FROM Candida orthopsilosi. INTERNATIONAL SEMINAR AND MINI WORKSHOP ON PHARMACEUTICS. School of Pharmacy ITB. Advanced Research in Pharmaceutical Science and Technology. 2015
13. Maharani Ugayadi, Marselina I Tan,Ernawati A Giri-Rahman , Fawzi R Zuhairi, Sony H Sumarsono. Primary monkey trachea cell culture express the subtantial components for influenza virus internalization. International Seminar on Tropical Bioresources for Sustainable Bioindustry. ITB Bandung, 30–31 October 2012
14. Regina Angeline, Rahma Tejawati Maryama,Ernawati Arifin Giri-Rachman and Wardono Niloperbowo. Mathematical Modelling of Growth of Recombinant Pichia pastoris , Producing sHBsAg, in Yeast Extract Potato Dextrose Medium (YEPD) and Buffered Glycerol-complex Medium (BMGY). International Conference on Mathematical and Natural Science . Institut Teknologi Bandung. 8-9 November 2012. Oral Presentation
15. Amy Estiati, Ade Nena,Ernawati A. Giri-Rachman , Putri Sandy Pangestu, Putri Dwi Utari, Ima Mulyama and Sony Suhandono. Transformation of Hepatitis B Surface Antigen into Local Banana Plants for Molecular Farming Vaccine. International Conference on Biomedical Science. Oral Poster Presentation. School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation.
16. Rahma Tejawati Maryama, Shinta Kusumawardhani, Dessy Natalia, Debbie S Retnoningrum andErnawati A. Giri-Rachman . Intracelullar Expression of HbsAg Genotype B in Pichia pastoris KM71 Hyperresistant Zeosin, International Conference on Biomedical Science . School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation.
17. Putri Dwi Utari, Dina Rachmi Ramadhani, FX Reymond Sutandy, Lailatul Badriyah, Tati Kristanti, Ami Estiati, Sony Suhandono andErnawati Arifin Giri-Rachman . Construction and Transient Expression of Recombinant Large Hepatitis B Surface Antigen (L-HBsAg) in Plants to Develop Third Generation Hepatitis B Vaccine. International Conference on Biomedical Science . School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation.

[/et_pb_accordion_item][et_pb_accordion_item title=” Lectures (last 5 years)”]

1. Basic Molecular and Cell Biology (For Microbiology Study Program)
2. Applied in Molecular and Cell Biology (For School of Pharmacy)
3. Diagnostic Microbiology
4. Microbial physiolgy (practical)
5. Microbial pathogenesis and Immunology
6. Project on Microbial Genetics
7. Virology
8. Current Issues in Biotechnology

[/et_pb_accordion_item][et_pb_accordion_item title=”Professional Organization”]

1. Society of Indonesian Microbiology, Bandung Branch Chairman Juni 2011

2. Society of Indonesian Microbiology, Bandung Branch Program Coordinator 2005-2011

3. Society of Indonesian Biochemistry and Molecular Biology, Bandung Branch Member 2011

[/et_pb_accordion_item][et_pb_accordion_item title=” Reviewer”]

Indonesian Torray Science Foundation Research Grant, 2006, 2008

[/et_pb_accordion_item][et_pb_accordion_item title=” Editor”]

Editorial Team : Microbiology Indonesia 2012

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